P27; KIP1 Monoklonaler Antikörper

P27; KIP1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

3F12C10

Kat-Nr. : 67355-1-Ig

Synonyme

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27KIP1

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  WB analysis of Jurkat using 67355-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67355-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67355-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67355-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

67355-1-Ig bindet in WB, ELISA P27; KIP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: P27; KIP1 fusion protein Ag14634
• Vollständiger Name: cyclin-dependent kinase inhibitor 1B (p27, Kip1)
• Berechnetes Molekulargewicht: 198 aa, 22 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC001971
• Gene symbol: p27 Kip1/CDKN1B
• Gene ID (NCBI): 1027
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

Publikationen

Species	Application	Title
human	WB	J Hepatocell Carcinoma; CYP2C8 Suppress Proliferation, Migration, Invasion and Sorafenib Resistance of Hepatocellular Carcinoma via PI3K/Akt/p27 kip1 Axis; Authors - Xin Zhou
human	WB	Cell Rep; FBXO38 mediates FGL1 ubiquitination and degradation to enhance cancer immunity and suppress inflammation; Authors - Tongguan Tian
mouse	WB	J Vasc Res; Isolation and Cultivation of Vascular Smooth Muscle Cells from the Mouse Circle of Willis; Authors - Wei Chang