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P27; KIP1 Monoklonaler Antikörper

P27; KIP1 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3F12C10

Kat-Nr. : 67355-1-PBS

Synonyme

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27; KIP1, P27KIP1

Filter:
  • Western Blot (WB) analysis of Jurkat cells using P27; KIP1 Monoclonal antibody (67355-1-Ig)

    WB analysis of Jurkat using 67355-1-Ig (same clone as 67355-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using P27; KIP1 Monoclonal antibody (67355-1-Ig)

    WB analysis of HSC-T6 using 67355-1-Ig (same clone as 67355-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using P27; KIP1 Monoclonal antibody (67355-1-Ig)

    WB analysis of NIH/3T3 using 67355-1-Ig (same clone as 67355-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using P27; KIP1 Monoclonal antibody (67355-1-Ig)

    WB analysis of K-562 using 67355-1-Ig (same clone as 67355-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67355-1-Ig (P27; KIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67355-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67355-1-PBS bindet in WB, Indirect ELISA P27; KIP1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen P27; KIP1 fusion protein Ag14634
Vollständiger Name cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Berechnetes Molekulargewicht 198 aa, 22 kDa
Beobachtetes Molekulargewicht 27 kDa
GenBank-ZugangsnummerBC001971
Gene symbol p27 Kip1/CDKN1B
Gene ID (NCBI) 1027
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.