P2RX3 Polyklonaler Antikörper

P2RX3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 17843-1-AP

Synonyme

ATP receptor, P2X purinoceptor 3, P2X3, Purinergic receptor

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  WB analysis of Jurkat using 17843-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17843-1-AP (P2RX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17843-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17843-1-AP (P2RX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 17843-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17843-1-AP (P2RX3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 17843-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17843-1-AP (P2RX3 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

17843-1-AP bindet in WB, IHC, IF, ELISA P2RX3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P2RX3 fusion protein Ag12207
• Vollständiger Name: purinergic receptor P2X, ligand-gated ion channel, 3
• Berechnetes Molekulargewicht: 397 aa, 44 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC074792
• Gene symbol: P2RX3
• Gene ID (NCBI): 5024
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Int Immunopharmacol; 4-Methylesculetin attenuates inflammatory pain via inhibition of Sp1-TRPV1 and inflammation related signaling pathways; Authors - Weixin Zhao
rat	WB	J Ethnopharmacol; The ethanol extract of Scutellaria baicalensis Georgi attenuates complete Freund's adjuvant (CFA)-induced inflammatory pain by suppression of P2X3 receptor; Authors - Li Gao
mouse	WB	Am J Physiol Cell Physiol; Regulatory function of praja ring finger ubiquitin ligase 2 mediated by the P2rx3/P2rx7 axis in mouse hippocampal neuronal cells.; Authors - Mengting Gong
rat	WB,IHC	Sleep Breath; Therapeutic effects and modulatory mechanism of Alpiniae oxyphyllae Fructus in chronic intermittent hypoxia induced enuresis in rats.; Authors - Miao-Shang Su
human	IHC	J Clin Med; Interstitial Cells of Cajal and P2X3 Receptors at Ureteropelvic Junction Obstruction and Their Relationship with Pain Response; Authors - Dominika Borselle
human	IHC	Transl Androl Urol; Evaluation of transurethral GreenLight laser-selective vaporization for refractory overactive bladder in women; Authors - Kun Du