P2RX4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

P2RX4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 13534-1-AP

Synonyme

Purinergic receptor, P2X4R, P2X4, P2X purinoceptor 4, ATP receptor

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  WB analysis of HeLa using 13534-1-AP

  WB result of P2RX4 antibody (13534-1-AP; 1:4000; room temperature for 1.5 hours) with sh-control and sh-P2RX4 transfected HeLa cells.

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  WB analysis of HeLa using 13534-1-AP

  WB result of P2RX4 antibody (13534-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and P2RX4 knockout HeLa cells.

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  IHC staining of human ovary cancer using 13534-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13534-1-AP (P2RX4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Produktinformation

13534-1-AP bindet in WB, IHC, IF, ELISA P2RX4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P2RX4 fusion protein Ag4445
• Vollständiger Name: purinergic receptor P2X, ligand-gated ion channel, 4
• Berechnetes Molekulargewicht: 388 aa, 43 kDa
• Beobachtetes Molekulargewicht: 45-60 kDa
• GenBank-Zugangsnummer: BC033826
• Gene symbol: P2RX4
• Gene ID (NCBI): 5025
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RX4 (P2X purinoceptor 4) is a receptor for ATP acting as a ligand gated ion channel. The calculated molecular weight of P2RX4 is 43 kDa, larger apparent molecular weights of 50-80 kDa have been reported, possibly due to post-translational glycosylation (PMID: 24040145, 15262999; 29382907; 12566439). The antibody can detect the band around 45 kDa.

Publikationen

Species	Application	Title
human		Autophagy; Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy; Authors - Kui Wang
mouse	WB	Sci Adv; α-Ketoglutaric acid ameliorates hyperglycemia in diabetes by inhibiting hepatic gluconeogenesis via serpina1e signaling.; Authors - Yexian Yuan ; KD Validated
human	IHC	Front Oncol; An Inflammatory Response-Related Gene Signature Can Impact the Immune Status and Predict the Prognosis of Hepatocellular Carcinoma.; Authors - Zhuo Lin
mouse	WB	Exp Neurol; Neuroprotective and neuro-rehabilitative effects of acute purinergic receptor P2X4 (P2X4R) blockade after ischemic stroke.; Authors - Pranay Srivastava
human	IF	CNS Neurosci Ther; Activation of P2X4 receptor exacerbates acute brain injury after intracerebral hemorrhage.; Authors - Si-Ting Wu
mouse	IHC	J Headache Pain; Microglia P2X4R-BDNF signalling contributes to central sensitization in a recurrent nitroglycerin-induced chronic migraine model.; Authors - Ting Long