P2RX7 Polyklonaler Antikörper

P2RX7 Polyklonal Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(21)

Kat-Nr. : 11144-1-AP

Synonyme

P2X7, Purinergic receptor

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  WB analysis using 11144-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11144-1-AP (P2RX7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of RAW 264.7 using 11144-1-AP

  1x10^6 RAW 264.7 cells were intracellularly stained with 0.5 ug P2RX7 Polyclonal antibody (11144-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Cerebellum-Gewebe
• Erfolgreiche Detektion in FC (Intra): RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

11144-1-AP bindet in WB, IHC, IF, FC (Intra), ELISA P2RX7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P2RX7 fusion protein Ag1600
• Vollständiger Name: purinergic receptor P2X, ligand-gated ion channel, 7
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC011913
• Gene symbol: P2RX7
• Gene ID (NCBI): 5027
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs) (PMID: 21724990). P2RX7 functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. P2RX7 is highly expressed by cells of the haemopoietic lineage and can mediate cell death, killing of infectious organisms, and regulation of the inflammatory response (PMID: 22102807).

Publikationen

Species	Application	Title
human	IF	J Immunol; The danger signal, extracellular ATP, is a sensor for an airborne allergen and triggers IL-33 release and innate Th2-type responses.; Authors - Kouzaki Hideaki H
mouse	WB	Front Cell Neurosci; Clemastine Alleviates Depressive-Like Behavior Through Reversing the Imbalance of Microglia-Related Pro-inflammatory State in Mouse Hippocampus.; Authors - Wen-Jun Su
		J Leukoc Biol; HMG-CoA reductase inhibitors activate caspase-1 in human monocytes depending on ATP release and P2X7 activation.; Authors - Liao Yi-Hsiang YH
rat	WB,IHC	Environ Toxicol; Astaxanthin Alleviates Lung Injury by Regulating Oxidative Stress, Inflammatory Response, P2X7 Receptor, NF-κB, Bcl-2, and Caspase-3 in LPS-Induced Endotoxemia; Authors - Seckin Ozkanlar
human	IF	J Exp Clin Cancer Res; Shock wave-induced ATP release from osteosarcoma U2OS cells promotes cellular uptake and cytotoxicity of methotrexate.; Authors - Baochang Qi
rat	WB,IHC	J Biochem Mol Toxicol; Blockade of P2X7 receptor-mediated purinergic signaling with A438079 protects against LPS-induced liver injury in rats; Authors - Adem Kara