P2RX7 Polyklonaler Antikörper

P2RX7 Polyklonal Antikörper für FC, IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 11144-1-AP

Synonyme

P2RX7, P2X7

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Galerie der Validierungsdaten

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  IHC staining of human prostate cancer using 11144-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11144-1-AP (P2RX7 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 11144-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11144-1-AP (P2RX7 Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human tonsillitis using 11144-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11144-1-AP (P2RX7 Antibody) at dilution of 1:400 (under 40x lens).

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  IF Staining of HeLa using 11144-1-AP

  Immunofluorescent analysis of HeLa cells using 11144-1-AP (P2RX7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 11144-1-AP

  1X10^6 HeLa cells were stained with 0.2ug P2RX7 antibody (11144-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Produktinformation

11144-1-AP bindet in WB, IHC, IF, FC, ELISA P2RX7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P2RX7 fusion protein Ag1600
• Vollständiger Name: purinergic receptor P2X, ligand-gated ion channel, 7
• Berechnetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC011913
• Gene symbol: P2RX7
• Gene ID (NCBI): 5027
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs) (PMID: 21724990). P2RX7 functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. P2RX7 is highly expressed by cells of the haemopoietic lineage and can mediate cell death, killing of infectious organisms, and regulation of the inflammatory response (PMID: 22102807).

Publikationen

Species	Application	Title
human	IF	J Immunol; The danger signal, extracellular ATP, is a sensor for an airborne allergen and triggers IL-33 release and innate Th2-type responses.; Authors - Kouzaki Hideaki H
mouse	WB	Front Cell Neurosci; Clemastine Alleviates Depressive-Like Behavior Through Reversing the Imbalance of Microglia-Related Pro-inflammatory State in Mouse Hippocampus.; Authors - Wen-Jun Su
		J Leukoc Biol; HMG-CoA reductase inhibitors activate caspase-1 in human monocytes depending on ATP release and P2X7 activation.; Authors - Liao Yi-Hsiang YH
human	IF	J Exp Clin Cancer Res; Shock wave-induced ATP release from osteosarcoma U2OS cells promotes cellular uptake and cytotoxicity of methotrexate.; Authors - Baochang Qi
rat	WB,IHC	J Biochem Mol Toxicol; Blockade of P2X7 receptor-mediated purinergic signaling with A438079 protects against LPS-induced liver injury in rats; Authors - Adem Kara
mouse	WB	Toxicol Lett; Maternal smoking during pregnancy aggravated muscle phenotype in FHL1-/y offspring mice similar to congenital clubfoot through P2RX7-mediated pyroptosis.; Authors - Yi Lou

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