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P53 Rekombinanter Antikörper
P53 Rekombinant Antikörper für WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Zebrafisch und mehr (1)
Anwendung
WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA
Konjugation
Unkonjugiert
CloneNo.
2H13
Kat-Nr. : 80077-1-RR
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | COLO 320-Zellen, A431-Zellen, HT-29-Zellen, ROS1728-Zellen, SW480-Zellen, Zebrafischgewebe | 
| Erfolgreiche Detektion in IHC-Autostainer | humanes Kolonkarzinomgewebe, human ovarian cancer | 
| Erfolgreiche Detektion in IF/ICC | HepG2-Zellen, A431-Zellen | 
| Erfolgreiche Detektion in FC (Intra) | HepG2-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 | 
| Immunhistochemie (IHC)-AUTOSTAINER | IHC-AUTOSTAINER : 1:50-1:500 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 | 
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 6 publications below | 
| IF | See 2 publications below | 
Produktinformation
80077-1-RR bindet in WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA P53 und zeigt Reaktivität mit human, Zebrafisch
| Getestete Reaktivität | human, Zebrafisch | 
| In Publikationen genannte Reaktivität | human, Maus, Zebrafisch | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Rekombinant | 
| Typ | Antikörper | 
| Immunogen | Peptid | 
| Vollständiger Name | tumor protein p53 | 
| Berechnetes Molekulargewicht | 44 kDa | 
| Beobachtetes Molekulargewicht | 53 kDa | 
| GenBank-Zugangsnummer | BC003596 | 
| Gene symbol | P53 | 
| Gene ID (NCBI) | 7157 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Protein-A-Reinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for P53 antibody 80077-1-RR | Protokoll herunterladen | 
| IHC protocol for P53 antibody 80077-1-RR | Protokoll herunterladenl | 
| IF protocol for P53 antibody 80077-1-RR | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Oncol Rep Pseudolaric acid B induces apoptosis associated with the mitochondrial and PI3K/AKT/mTOR pathways in triple‑negative breast cancer | ||
| Neurotox Res NRF2 Antagonizes HIV-1 Tat and Methamphetamine-Induced BV2 Cell Ferroptosis by Regulating SLC7A11 | ||
| Acta Biochim Biophys Sin (Shanghai) Hydrogen sulfide ameliorates senescence in vascular endothelial cells through ameliorating inflammation and activating PPARδ/SGLT2/STAT3 signaling pathway | ||
| Cell Biol Toxicol Deficiency of copper responsive gene stmn4 induces retinal developmental defects | ||
| World J Gastroenterol Silent information regulator sirtuin 1 ameliorates acute liver failure via the p53/glutathione peroxidase 4/gasdermin D axis | 












