P53 Rekombinanter Antikörper

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• KD/KO Validated

P53 Rekombinant Antikörper für WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Zebrafisch und mehr (1)

Anwendung

WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

2H13

Kat-Nr. : 80077-1-RR

Synonyme

TP53, 2H13, Antigen NY-CO-13, tumor protein p53, Tumor suppressor p53

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  WB analysis using 80077-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of zebrafish using 80077-1-RR

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 80077-1-RR

  WB result of P53 antibody (80077-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P53 transfected A431 cells.

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  WB analysis of A431 using 80077-1-RR

  A431 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of SW480 using 80077-1-RR

  SW480 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-29 using 80077-1-RR

  HT-29 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 80077-1-RR

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovarian cancer using 80077-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue slide using 80077-1-RR (p53 antibody) at dilution of 1:1000 (under 10x lens).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 7.0). Staining was performed on the Epredia 480S Autostainer.​

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  IHC staining of human ovarian cancer using 80077-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue slide using 80077-1-RR (p53 antibody) at dilution of 1:1000 (under 40x lens).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 7.0). Staining was performed on the Epredia 480S Autostainer.

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  IHC staining of human colon cancer using 80077-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80077-1-RR (P53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 80077-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 80077-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

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  FC experiment of HepG2 using 80077-1-RR

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human P53 (80077-1-RR, Clone:2H13) (red) labeled with FlexAble CoraLite®488 Antibody Labeling Kit for Rabbit IgG (KFA001), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, A431-Zellen, HT-29-Zellen, ROS1728-Zellen, SW480-Zellen, Zebrafischgewebe
• Erfolgreiche Detektion in IHC-Autostainer: humanes Kolonkarzinomgewebe, human ovarian cancer
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, A431-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC)-AUTOSTAINER: IHC-AUTOSTAINER : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 2 publications below

Produktinformation

80077-1-RR bindet in WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA P53 und zeigt Reaktivität mit human, Zebrafisch

• Getestete Reaktivität: human, Zebrafisch
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tumor protein p53
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC003596
• Gene symbol: P53
• Gene ID (NCBI): 7157
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)

Publikationen

Species	Application	Title
human	WB	Oncol Rep; Pseudolaric acid B induces apoptosis associated with the mitochondrial and PI3K/AKT/mTOR pathways in triple‑negative breast cancer; Authors - Ke Yang
mouse	WB,IF	Neurotox Res; NRF2 Antagonizes HIV-1 Tat and Methamphetamine-Induced BV2 Cell Ferroptosis by Regulating SLC7A11; Authors - Shucheng Lin
human	WB	Acta Biochim Biophys Sin (Shanghai); Hydrogen sulfide ameliorates senescence in vascular endothelial cells through ameliorating inflammation and activating PPARδ/SGLT2/STAT3 signaling pathway; Authors - Danyang Tian
zebrafish	WB	Cell Biol Toxicol; Deficiency of copper responsive gene stmn4 induces retinal developmental defects; Authors - YuanYuan Jing
mouse	WB	Reprod Toxicol; Cadmium disrupt spermatogenic cell cycle via piRNA-DQ717867/p53 pathway; Authors - Jiaoyang Wei
mouse	WB,IF	World J Gastroenterol; Silent information regulator sirtuin 1 ameliorates acute liver failure via the p53/glutathione peroxidase 4/gasdermin D axis; Authors - Xing-Nian Zhou