DDX6 Polyklonaler Antikörper

DDX6 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 14632-1-AP

Synonyme

ATP dependent RNA helicase p54, DDX6, DEAD box protein 6, HLR2, Oncogene RCK, P54, RCK

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Galerie der Validierungsdaten

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  WB analysis using 14632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14632-1-AP (DDX6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14632-1-AP (DDX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14632-1-AP

  IP Result of anti-DDX6 (IP:14632-1-AP, 4ug; Detection:14632-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human breast cancer using 14632-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14632-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14632-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14632-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using 14632-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DDX6 antibody (14632-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 14632-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DDX6 antibody (14632-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C2C12-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 3 publications below

Produktinformation

14632-1-AP bindet in WB, IP, IHC, IF, ELISA DDX6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX6 fusion protein Ag6211
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC065007
• Gene symbol: DDX6
• Gene ID (NCBI): 1656
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

P54, also named as DDX6, HLR2, and RCK, is a 483 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and belongs to the DEAD box helicase family. DDX6/DHH1 subfamily. P54 is abundantly expressed in most tissues. In the process of mRNA degradation, P54 may play a role in mRNA decapping. P54 is involved in the replication of hepatitis C virus genomes in hepatocytes and in tumourigenesis of hepatocellular carcinomas.

Publikationen

Species	Application	Title
mouse	WB,IF	J Genet Genomics; LSM14B coordinates protein component expression in the P-body and controls oocyte maturation; Authors - Huiru Zhang
human	WB,IF	iScience; The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.; Authors - Limei Zou
mouse	WB	Biol Reprod; Translational Activation of Developmental Messenger RNAs During Neonatal Mouse Testis Development.; Authors - Chappell Vesna A VA
mouse	IF	BMC Bioinformatics; SpotitPy: a semi-automated tool for object-based co-localization of fluorescent labels in microscopy images; Authors - Alexia Akalestou-Clocher
mouse	WB	Int J Legal Med; Candidate biomarkers in brown adipose tissue for post-mortem diagnosis of fatal hypothermia; Authors - Miao Zhang
human	WB	Sci Adv; PARP14 is a PARP with both ADP-ribosyl transferase and hydrolase activities; Authors - Nina Đukić