DDX6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DDX6 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Rezensionen (1)

Kat-Nr. : 14632-1-AP

Synonyme

ATP-dependent RNA helicase p54, EC:3.6.4.13, HLR2, P54, Probable ATP-dependent RNA helicase DDX6

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  WB analysis using 14632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14632-1-AP (DDX6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14632-1-AP (DDX6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14632-1-AP (DDX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14632-1-AP

  IP result of anti-DDX6 (IP:14632-1-AP, 4ug; Detection:14632-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human breast cancer using 14632-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14632-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14632-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14632-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14632-1-AP (DDX6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of hTERT-RPE1 using 14632-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DDX6 antibody (14632-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 14632-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DDX6 antibody (14632-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C2C12-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IF: See 7 publications below

Produktinformation

14632-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA DDX6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX6 fusion protein Ag6211
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC065007
• Gene symbol: DDX6
• Gene ID (NCBI): 1656
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX6, also named as P54, HLR2, and RCK, is a 483 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and belongs to the DEAD box helicase family. DDX6/DHH1 subfamily. P54 is abundantly expressed in most tissues. In the process of mRNA degradation, P54 may play a role in mRNA decapping. P54 is involved in the replication of hepatitis C virus genomes in hepatocytes and in tumourigenesis of hepatocellular carcinomas.

Publikationen

Species	Application	Title
	WB,IF	Adv Sci (Weinh); Primate-Specific DAZ Regulates Translation of Cell Proliferation-Related mRNAs and is Essential for Maintenance of Spermatogonia; Authors - Ningjing Ou
mouse	WB,IF	J Genet Genomics; LSM14B coordinates protein component expression in the P-body and controls oocyte maturation; Authors - Huiru Zhang
human	WB,IF	iScience; The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.; Authors - Limei Zou
mouse	WB	Biol Reprod; Translational Activation of Developmental Messenger RNAs During Neonatal Mouse Testis Development.; Authors - Chappell Vesna A VA
human	WB	Biochem Biophys Res Commun; DDX6 is involved in the pathogenesis of inflammatory diseases via NF-κB activation; Authors - Seiichiro Naito ; KD Validated
human	WB	EMBO J; Energy stress promotes P-bodies formation via lysine-63-linked polyubiquitination of HAX1; Authors - Wanqi Zhan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-13-2024)

The antibody worked well to detect DDX6 in HeLa cells by WB (1/1000 dilution, Overnight incubation at 4°C) and by Immunofluorescence to observe P-Bodies (1h incubation at RT - 1/500).

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa