P5CS Monoklonaler Antikörper

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• KD/KO Validated

P5CS Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3B1E11

Kat-Nr. : 68184-1-Ig

Synonyme

ALDH18A1, 3B1E11, Aldehyde dehydrogenase family 18 member A1, Delta-1-pyrroline-5-carboxylate synthase, Gamma-glutamyl kinase

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  WB analysis using 68184-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68184-1-Ig (P5CS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HAP1 using 68184-1-Ig

  WB result of P5CS antibody (68184-1-Ig; 1:10000; room temperature for 1.5 hours) with wild-type and sg-P5CS transfected HAP1 cells.

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  WB analysis of mouse colon using 68184-1-Ig

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 68184-1-Ig (P5CS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat colon using 68184-1-Ig

  rat colon tissue were subjected to SDS PAGE followed by western blot with 68184-1-Ig (P5CS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, LNCaP-Zellen, MCF-7-Zellen, Maus-Kolongewebe, Ratten-Kolongewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

68184-1-Ig bindet in WB, IF, ELISA P5CS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: P5CS fusion protein Ag12100
• Vollständiger Name: aldehyde dehydrogenase 18 family, member A1
• Berechnetes Molekulargewicht: 795 aa, 87 kDa
• Beobachtetes Molekulargewicht: 87 kDa
• GenBank-Zugangsnummer: BC106054
• Gene symbol: P5CS
• Gene ID (NCBI): 5832
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS, P5CS, PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse	IF	Nature; Cellular ATP demand creates metabolically distinct subpopulations of mitochondria; Authors - Keun Woo Ryu