P62,SQSTM1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

P62,SQSTM1 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D17

Kat-Nr. : 80294-1-PBS

Synonyme

P62/SQSTM1, P62, SQSTM1, Sequestosome-1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa

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  WB analysis using 80294-1-RR (same clone as 80294-1-PBS)

  Lysates of HepG2 cells treated with Chloroquine or not were subjected to SDS PAGE followed by western blot with 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 80294-1-RR (same clone as 80294-1-PBS)

  WB result of P62,SQSTM1 antibody (80294-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62,SQSTM1 transfected HEK-293 cells. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

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  WB analysis using 80294-1-RR (same clone as 80294-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 80294-1-RR (same clone as 80294-1-PBS)

  WB result of P62,SQSTM1 antibody (80294-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62,SQSTM1 transfected HepG2 cells. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 80294-1-RR (same clone as 80294-1-PBS)

  IP result of anti-P62,SQSTM1 (IP:80294-1-RR, 4ug; Detection:80294-1-RR 1:500) with HEK-293 cells lysate 1640 ug. This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 80294-1-RR (same clone as 80294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 80294-1-RR (same clone as 80294-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80294-1-RR (same clone as 80294-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using P62,SQSTM1 antibody (80294-1-RR, Clone: 1D17 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 80294-1-RR (same clone as 80294-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human P62,SQSTM1 (80294-1-RR, Clone:1D17) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80294-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80294-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA P62,SQSTM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: P62,SQSTM1 fusion protein Ag13131
• Vollständiger Name: sequestosome 1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC017222
• Gene symbol: P62/SQSTM1
• Gene ID (NCBI): 8878
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease, and nephropathic cystinosis (PMID: 22074114, 22860231, 22714671).