P62/SQSTM1 Rekombinanter Antikörper

P62/SQSTM1 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

241992C4

Kat-Nr. : 84826-1-RR

Synonyme

p62, Sqstm1, 241992C4, OSF 6, Osi

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  WB analysis using 84826-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 84826-1-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84826-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 84826-1-RR

  IP result of anti-P62/SQSTM1 (IP:84826-1-RR, 4ug; Detection:84826-1-RR 1:4000) with NIH/3T3 cells lysate 1920 ug.

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  IHC staining of mouse brain using 84826-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84826-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84826-1-RR (P62/SQSTM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH/3T3 using 84826-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Chloroquine treated NIH/3T3 cells using P62,SQSTM1 antibody (84826-1-RR, Clone: 241992C4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of NIH/3T3 using 84826-1-RR

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug P62/SQSTM1 Recombinant antibody (84826-1-RR, Clone:241992C4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84826-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84826-1-RR against Mouse P62,SQSTM1 were performed. The affinity constant is 0.203 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, Maushirngewebe, Mauslebergewebe, Raji-Zellen, Rattenhirngewebe
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Chloroquine treated NIH/3T3 cells
• Erfolgreiche Detektion in FC (Intra): NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

84826-1-RR bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA P62/SQSTM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: P62/SQSTM1 fusion protein Ag35064
• Vollständiger Name: sequestosome 1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: NM_011018
• Gene symbol: P62/SQSTM1
• Gene ID (NCBI): 18412
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer.

Publikationen

Species	Application	Title
mouse	WB,IF	Toxicology; Chronic exposure to polystyrene microplastics induces renal fibrosis via ferroptosis; Authors - Runyang Hong