- Featured Product
- KD/KO Validated
P62/SQSTM1 Monoklonaler Antikörper
P62/SQSTM1 Monoklonal Antikörper für IF/ICC
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human
Anwendung
IF/ICC
Konjugation
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1H5C1
Kat-Nr. : CL488-66184
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in IF/ICC | HepG2-Zellen, mit Chloroquin behandelte U2OS-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Produktinformation
CL488-66184 bindet in IF/ICC P62/SQSTM1 und zeigt Reaktivität mit human
| Getestete Reaktivität | human | 
| Wirt / Isotyp | Maus / IgG2a | 
| Klonalität | Monoklonal | 
| Typ | Antikörper | 
| Immunogen | P62/SQSTM1 fusion protein Ag13131 | 
| Vollständiger Name | sequestosome 1 | 
| Berechnetes Molekulargewicht | 48 kDa | 
| Beobachtetes Molekulargewicht | 62 kDa | 
| GenBank-Zugangsnummer | BC017222 | 
| Gene symbol | P62/SQSTM1 | 
| Gene ID (NCBI) | 8878 | 
| Konjugation | CoraLite® Plus 488 Fluorescent Dye | 
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm | 
| Form | Liquid | 
| Reinigungsmethode | Protein-A-Reinigung | 
| Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA | 
| Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| IF protocol for CL Plus 488 P62/SQSTM1 antibody CL488-66184 | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 


