PA2G4 Monoklonaler Antikörper

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• KD/KO Validated

PA2G4 Monoklonal Antikörper für WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3G10D9

Kat-Nr. : 66055-1-PBS

Synonyme

EBP1, ErbB3 binding protein 1, HG4 1, p38 2G4, PA2G4

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  WB analysis using 66055-1-Ig (same clone as 66055-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66055-1-Ig (PA2G4 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66055-1-Ig (same clone as 66055-1-PBS)

  WB result of PA2G4 antibody (66055-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PA2G4 transfected HEK-293 cells. This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50029-1

  Sandwich ELISA standard curve of MP50029-1, PA2G4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66055-2-PBS. Detection antibody: HRP-conjugated 66055-1-PBS. Standard: Ag7850. Range: 1.25-20 ng/mL.

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  IHC staining of human breast cancer using 66055-1-Ig (same clone as 66055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66055-1-Ig (PA2G4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66055-1-Ig (same clone as 66055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66055-1-Ig (PA2G4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66055-1-Ig (same clone as 66055-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PA2G4 antibody (66055-1-Ig, Clone: 3G10D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66055-1-PBS bindet in WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA PA2G4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PA2G4 fusion protein Ag7850
• Vollständiger Name: proliferation-associated 2G4, 38kDa
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 38-42 kDa, 48 kDa
• GenBank-Zugangsnummer: BC001951
• Gene symbol: PA2G4
• Gene ID (NCBI): 5036
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a mouse mono-clonal antibody raised against full length PA2G4 of human origin.