PAAF1 Polyklonaler Antikörper

PAAF1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17650-1-PBS

Synonyme

WDR71, WD repeat-containing protein 71, Rpn14, Protein G-16, Proteasomal ATPase-associated factor 1

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  WB analysis of Jurkat using 17650-1-AP (same clone as 17650-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 17650-1-AP (same clone as 17650-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 17650-1-AP (same clone as 17650-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 17650-1-AP (same clone as 17650-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 17650-1-AP (same clone as 17650-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17650-1-AP (PAAF1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 17650-1-AP (same clone as 17650-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PAAF1 antibody (17650-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17650-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PAAF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAAF1 fusion protein Ag11892
• Vollständiger Name: proteasomal ATPase-associated factor 1
• Berechnetes Molekulargewicht: 375 aa, 40 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC028628
• Gene symbol: PAAF1
• Gene ID (NCBI): 80227
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAAF1(Proteasomal ATPase-associated factor 1) is also named as WDR71 and belongs to the WD repeat PAAF1/RPN14 family. It functions as a negative regulator of the proteasome by controlling the assembly/disassembly of the proteasome. PAAF1 has 3 isoforms produced by alternative splicing.