PABPC4 Monoklonaler Antikörper

PABPC4 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E6B3

Kat-Nr. : 68187-1-PBS

Synonyme

Activated platelet protein 1, APP 1, APP1, FLJ43938, iPABP, PABP 4, PABP4, PABPC4, Poly(A) binding protein 4

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  WB analysis using 68187-1-Ig (same clone as 68187-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68187-1-Ig (PABPC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68187-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 68187-1-Ig (same clone as 68187-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68187-1-Ig (PABPC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68187-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 68187-1-Ig (same clone as 68187-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68187-1-Ig (PABPC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68187-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68187-1-Ig (same clone as 68187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68187-1-Ig (PABPC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68187-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68187-1-Ig (same clone as 68187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68187-1-Ig (PABPC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68187-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 68187-1-Ig (same clone as 68187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68187-1-Ig (PABPC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68187-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 68187-1-Ig (same clone as 68187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68187-1-Ig (PABPC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68187-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68187-1-Ig (same clone as 68187-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PABPC4 antibody (68187-1-Ig, Clone: 1E6B3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68187-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68187-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PABPC4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PABPC4 fusion protein Ag6813
• Vollständiger Name: poly(A) binding protein, cytoplasmic 4 (inducible form)
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 71 kDa
• GenBank-Zugangsnummer: BC065540
• Gene symbol: PABPC4
• Gene ID (NCBI): 8761
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Poly(A)-binding protein (PABP), a nucleocytoplasmic shuttling protein, has a fundamental role in promoting gene expression by enhancing mRNA translation and stability(PMID: 21940797). Poly(A)-binding protein 4 (PABPC4), one of the homologs of PABP, was initially identified as a human T-cell activation-induced protein. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules(NCBI).