PABPN1 Monoklonaler Antikörper

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• KD/KO Validated

PABPN1 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3C12E12

Kat-Nr. : 66807-1-PBS

Synonyme

3C12E12, Nuclear poly(A)-binding protein 1, PAB2, PABII, PABP2

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  WB analysis using 66807-1-Ig (same clone as 66807-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66807-1-Ig (PABPN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66807-1-Ig (same clone as 66807-1-PBS)

  WB result of PABPN1 antibody (66807-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PABPN1 transfected HeLa cells. This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

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  WB analysis using 66807-1-Ig (same clone as 66807-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66807-1-Ig (PABPN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 66807-1-Ig (same clone as 66807-1-PBS)

  IP result of anti-PABPN1 (IP:66807-1-Ig, 5ug; Detection:66807-1-Ig 1:600) with Jurkat cells lysate 1680 ug. This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66807-1-Ig (same clone as 66807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 66807-1-Ig (same clone as 66807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 66807-1-Ig (same clone as 66807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66807-1-Ig (same clone as 66807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 66807-1-Ig (same clone as 66807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66807-1-Ig (same clone as 66807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66807-1-Ig (same clone as 66807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66807-1-Ig (same clone as 66807-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66807-1-Ig (PABPN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66807-1-Ig (same clone as 66807-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PABPN1 antibody (66807-1-Ig, Clone: 3C12E12 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66807-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66807-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PABPN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PABPN1 fusion protein Ag27470
• Vollständiger Name: poly(A) binding protein, nuclear 1
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 50-56 kDa
• GenBank-Zugangsnummer: BC010939
• Gene symbol: PABPN1
• Gene ID (NCBI): 8106
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Poly(A) Binding Protein Nuclear 1(PABPN1) is one of the isoforms of poly(A) binding protein (PABP) that locating to the nucleus in eukaryotic cells. And PABPN1 plays important role in mRNA stability by regulating of poly-A tail length, mRNA decay and proximal polyadenylation site (PAS) utilization at the 3'-UTR (PMID:23300856). Another report showed that PABPN1 shuttles between the nucleus and the cytoplasm to export poly(A) RNA from the nucleus (PMID:23601051). It is reported that PABPN1 is ubiquitously expressed in almost all tissues, however, mutations in PABPN1 causes Oculopharyngeal Muscular Dystrophy (OPMD) which affected in skeletal muscles specifically. Because of a mild reduction in PABPN1 levels is sufficient to induce muscle wasting (PMID:27152426). Acetylation, Methylation, Phosphoprotein are common post-translational modifications of PABPN1 protein. And 50-55 kDa bands have been reported (PMID: 29939290; 28361972).