PACS2 Polyklonaler Antikörper

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• KD/KO Validated

PACS2 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30172-1-PBS

Synonyme

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  WB analysis using 30172-1-AP (same clone as 30172-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30172-1-AP (PACS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 30172-1-AP (same clone as 30172-1-PBS)

  WB result of PACS2 antibody (30172-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACS2 transfected HCT 116 cells. This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30172-1-AP (same clone as 30172-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30172-1-AP (PACS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

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  IP experiment of HCT 116 using 30172-1-AP (same clone as 30172-1-PBS)

  IP result of anti-PACS2 (IP:30172-1-AP, 4ug; Detection:30172-1-AP 1:1500) with HCT 116 cells lysate 2440 ug. This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

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  IF Staining of HCT 116 using 30172-1-AP (same clone as 30172-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using PACS2 antibody (30172-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30172-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA PACS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PACS2 fusion protein Ag32902
• Vollständiger Name: phosphofurin acidic cluster sorting protein 2
• Berechnetes Molekulargewicht: 98kd
• Beobachtetes Molekulargewicht: 100-130 kDa
• GenBank-Zugangsnummer: NM_001100913
• Gene symbol: PACS2
• Gene ID (NCBI): 23241
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PACS2, also named PACS1L or KIAA0602, is one of PACS (phosphofurin acidic cluster sorting) proteins which are one such family of multifunctional membrane traffic regulators that mediate organ homoeostasis and have important roles in diverse pathologies and disease states. PACS2 is a multifunctional sorting protein that controls the endoplasmic reticulum (ER)-mitochondria communication, ER homeostasis, and apoptosis. PACS2 may be involved in ion channel trafficking, directing acidic cluster-containing ion channels to distinct subcellular compartments.