PACSIN1 Polyklonaler Antikörper

PACSIN1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 13219-1-AP

Synonyme

KIAA1379, PACSIN1, SDPI

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Galerie der Validierungsdaten

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  WB analysis of mouse cerebellum using 13219-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13219-1-AP (PACSIN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13219-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13219-1-AP (PACSIN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 13219-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13219-1-AP (PACSIN1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 13219-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13219-1-AP (PACSIN1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 13219-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13219-1-AP (PACSIN1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 13219-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13219-1-AP (PACSIN1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of SH-SY5Y using 13219-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 13219-1-AP (PACSIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 13219-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 13219-1-AP (PACSIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

13219-1-AP bindet in WB, IHC, IF, ELISA PACSIN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PACSIN1 fusion protein Ag4102
• Vollständiger Name: protein kinase C and casein kinase substrate in neurons 1
• Berechnetes Molekulargewicht: 444 aa, 51 kDa
• Beobachtetes Molekulargewicht: 48-51 kDa
• GenBank-Zugangsnummer: BC040228
• Gene symbol: PACSIN1
• Gene ID (NCBI): 29993
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PACSIN1 (also known as syndapin-1) is a member of the protein kinase C and casein kinase substrate in neurons (PACSIN) family. In mammals, the PACSIN family is comprised of three members, PACSIN1, PACSIN2, and PACSIN3 (PMID: 34990060). PACSIN1 is expressed mainly in neurons, whereas PACSIN2 is ubiquitously expressed in all tissues, and PACSIN3 is expressed mainly in skeletal muscle and the heart (PMID: 23668323). All of these three members contain an N-terminal F-BAR domain and a C-terminal SH3 domain. PACSIN1 plays a role in endocytosis and endosomal recycling. Meanwhile, it has a role in actin remodeling and microtubule nucleation and also plays a particular role in membrane shaping and reconstruction (PMID: 23035120; 34422904). PACSIN1 is involved in neuromorphogenesis and the regulation of the nervous system, and the inappropriate expression of PACSIN1 has been associated with some neurological diseases, including schizophrenia, Alzheimer's disease, and Huntington's disease (PMID: 34990060).

Publikationen

Species	Application	Title
human	IHC	Front Mol Biosci; Decreased Expression of PACSIN1 in Brain Glioma Samples Predicts Poor Prognosis.; Authors - Zhou Zimu