PADI2 Monoklonaler Antikörper

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PADI2 Monoklonal Antikörper für WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D8C12

Kat-Nr. : 66386-1-PBS

Synonyme

EC:3.5.3.15, KIAA0994, PAD H19, PAD2, PAD-H19

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  WB analysis of MCF-7 using 66386-1-Ig (same clone as 66386-1-PBS)

  WB result of PADI2 antibody (66386-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected MCF-7 cells. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 66386-1-Ig (same clone as 66386-1-PBS)

  human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66386-1-Ig (same clone as 66386-1-PBS)

  WB result of PADI2 antibody (66386-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected HeLa cells. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66386-1-Ig (same clone as 66386-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66386-1-Ig (same clone as 66386-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66386-1-Ig (same clone as 66386-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66386-1-Ig (PADI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66386-1-Ig (same clone as 66386-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66386-1-Ig (PADI2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66386-1-Ig (same clone as 66386-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66386-1-Ig (PADI2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66386-1-Ig (same clone as 66386-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66386-1-Ig (PADI2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66386-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50376-2

  Cytometric bead array standard curve of MP50376-2, PADI2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66386-1-PBS. Detection antibody: 66386-3-PBS. Standard:Ag17612. Range: 0.781-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66386-1-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA PADI2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PADI2 fusion protein Ag17612
• Vollständiger Name: peptidyl arginine deiminase, type II
• Berechnetes Molekulargewicht: 665 aa, 75 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC009701
• Gene symbol: PADI2
• Gene ID (NCBI): 11240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PADI2, also named as KIAA0994, PDI2, PAD-H19 and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.