PAH Polyklonaler Antikörper

PAH Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 16347-1-AP

Synonyme

PAH, PH, Phe 4 monooxygenase, Phenylalanine 4 hydroxylase, phenylalanine hydroxylase, PKU, PKU1

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 16347-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16347-1-AP (PAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16347-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16347-1-AP (PAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16347-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16347-1-AP (PAH Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 16347-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16347-1-AP (PAH Antibody) at dilution of 1:200 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

16347-1-AP bindet in WB, IHC, ELISA PAH und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAH fusion protein Ag9541
• Vollständiger Name: phenylalanine hydroxylase
• Berechnetes Molekulargewicht: 452 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC026251
• Gene symbol: PAH
• Gene ID (NCBI): 5053
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAH, also known as PH, belongs to the aromatic amino acid hydroxylase (AAAH) enzyme family. PAH catalyzes the conversion of L-phenylalanine (L-Phe) to L-tyrosine (L-Tyr) by para-hydroxylation of the aromatic side chain. PAH is primarily present in the liver, where removal of excess L-Phe prevents the neurotoxic effect of hyperphenylalaninemia (HPA). The calculated molecular weight of PAH is 52 kDa (PMID: 23457044).

Publikationen

Species	Application	Title
mouse	WB	Pharmacol Res; Medioresinol as a novel PGC-1α activator prevents pyroptosis of endothelial cells in ischemic stroke through PPARα-GOT1 axis.; Authors - Yunjie Wang
mouse	WB	Life Sci Alliance; A phosphoproteomic approach reveals that PKD3 controls PKA-mediated glucose and tyrosine metabolism.; Authors - Angel Loza-Valdes
human	WB	Adv Sci (Weinh); A Metabolic Reprogramming Amino Acid Polymer as an Immunosurveillance Activator and Leukemia Targeting Drug Carrier for T‐Cell Acute Lymphoblastic Leukemia; Authors - Changzheng Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Angel (Verified Customer) (11-27-2020)

This antibody detects PAH in liver tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver tissue

PAH Antibody Western Blot validation (1:1000 dilution) in Liver tissue (Cat no:16347-1-AP)