PAH Rekombinanter Antikörper

PAH Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251007A9

Kat-Nr. : 86231-2-PBS

Synonyme

EC:1.14.16.1, PH, Phe-4-monooxygenase, Phenylalanine-4-hydroxylase, PKU

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  WB analysis using 86231-2-RR (same clone as 86231-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86231-2-RR (PAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86231-2-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 86231-2-RR (same clone as 86231-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 86231-2-RR (PAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86231-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 86231-2-RR (same clone as 86231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86231-2-RR (PAH antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86231-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 86231-2-RR (same clone as 86231-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 86231-2-RR (PAH antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86231-2-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02346-1

  The mean PAH concentration was determined to be 89.5 ng/mL in Hela cell extract based on a 0.9 mg/mL extract load.

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  Cytometric bead array standard curve of MP02346-1

  Cytometric bead array standard curve of MP02346-1, PAH Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86231-2-PBS. Detection antibody: 86231-1-PBS. Standard: Ag9541. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 86231-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86231-2-RR against Human PAH were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

86231-2-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA PAH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PAH fusion protein Ag9541
• Vollständiger Name: phenylalanine hydroxylase
• Berechnetes Molekulargewicht: 452 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC026251
• Gene symbol: PAH
• Gene ID (NCBI): 5053
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAH, also known as PH, belongs to the aromatic amino acid hydroxylase (AAAH) enzyme family. PAH catalyzes the conversion of L-phenylalanine (L-Phe) to L-tyrosine (L-Tyr) by para-hydroxylation of the aromatic side chain. PAH is primarily present in the liver, where removal of excess L-Phe prevents the neurotoxic effect of hyperphenylalaninemia (HPA). The calculated molecular weight of PAH is 52 kDa (PMID: 23457044).