PAICS Rekombinanter Antikörper

PAICS Rekombinant Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250910H8

Kat-Nr. : 86255-2-PBS

Synonyme

ADE2, ADE2H1, AIR carboxylase, AIRC, Bifunctional phosphoribosylaminoimidazole carboxylase/phosphoribosylaminoimidazole succinocarboxamide synthetase

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  WB analysis using 86255-2-RR (same clone as 86255-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86255-2-RR (PAICS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86255-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02343-2

  Sandwich ELISA standard curve of MP02343-2, Human PAICS Recombinant Matched Antibody Pair - PBS only. 86255-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3621. 86255-4-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL

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  Sample test of MP02343-1

  The mean PAICS concentration was determined to be 91.8 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

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  Sample test of MP02343-2

  The mean PAICS concentration was determined to be 62.65 ng/mL in THP-1 cell extract based on a 1.2 mg/mL extract load.

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  IHC staining of human hepatocellular cancer using 86255-2-RR (same clone as 86255-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 86255-2-RR (PAICS antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86255-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86255-2-RR (same clone as 86255-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PAICS antibody (86255-2-RR, Clone: 250910H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86255-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02343-1

  Cytometric bead array standard curve of MP02343-1, PAICS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86255-2-PBS. Detection antibody: 86255-1-PBS. Standard: Ag3621. Range: 0.469-60 ng/mL.

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  Affinity and Kinetic Characterization of 86255-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86255-2-RR against Human PAICS were performed. The affinity constant is 0.357 nM.

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Geprüfte Anwendungen

Produktinformation

86255-2-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test PAICS und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PAICS fusion protein Ag3621
• Vollständiger Name: phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase
• Berechnetes Molekulargewicht: 425 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC010273
• Gene symbol: PAICS
• Gene ID (NCBI): 10606
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAICS is also named as ADE2(Multifunctional protein), AIRC, PAIS. It is an important bifunctional enzyme in de novo purine biosynthesis in vertebrate with both 5-aminoimidazole ribonucleotide carboxylase (AIRc) and 4-(N-succinylcarboxamide)-5-aminoimidazole ribonucleotide synthetase (SAICARs) activities. It becomes an attractive target for rational anticancer drug design, since rapidly dividing cancer cells rely heavily on the purine de novo pathway for synthesis of adenine and guanine, whereas normal cells favor the salvage pathway(PMID:17224163).