PAIP1 Monoklonaler Antikörper

PAIP1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Kaninchen, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H9D4

Kat-Nr. : 68042-1-PBS

Synonyme

PABP interacting protein 1, PAIP 1, PAIP1

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  WB analysis using 68042-1-Ig (same clone as 68042-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

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  WB analysis using 68042-1-Ig (same clone as 68042-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit brain using 68042-1-Ig (same clone as 68042-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 68042-1-Ig (same clone as 68042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68042-1-Ig (PAIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68042-1-PBS bindet in WB, IHC, Indirect ELISA PAIP1 und zeigt Reaktivität mit human, Kaninchen, Maus

• Getestete Reaktivität: human, Kaninchen, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PAIP1 fusion protein Ag16773
• Vollständiger Name: poly(A) binding protein interacting protein 1
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 40-45kDa, 62kDa
• GenBank-Zugangsnummer: BC005295
• Gene symbol: PAIP1
• Gene ID (NCBI): 10605
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAIP1 is a PABP-binding protein that contains two distinct PABP-binding motifs (PAMs). PAM1 binds to RNA recognition motif 2 in the N terminus of PABP; PAM2, which is a conserved region comprising approximately 15 amino acids, binds to the PABC domain of PABP. PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.