PAK2 Polyklonaler Antikörper

PAK2 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 19979-1-AP

Synonyme

C t PAK2, Gamma PAK, p21 activated kinase 2, p27 PAK 2p34, p34, p58, PAK 2, PAK2, PAK65, PAKgamma, S6/H4 kinase

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Galerie der Validierungsdaten

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  WB analysis using 19979-1-AP

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 19979-1-AP (PAK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19979-1-AP (PAK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 19979-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19979-1-AP (PAK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 19979-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PAK2 antibody (19979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Skelettmuskelgewebe, Jurkat-Zellen, PC-3-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19979-1-AP bindet in WB, IF,ELISA PAK2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: p21 protein (Cdc42/Rac)-activated kinase 2
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: NM_002577
• Gene symbol: PAK2
• Gene ID (NCBI): 5062
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAK2, also named as PAK65, PAKgamma, p58, PAK-2p27, PAK-2p24 and C-t-PAK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. PAK2 has several isoforms with the MW of 54-62 kDa and 41 kDa. The 62 kDa PAK2 is cleaved into a 34 kDa C terminal fragment and a 28 kDa N terminal fragment with a time course that parallels apoptotic death in Jurkat cells. (PMID:10200518). Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form. The antibody has no cross reaction to PAK1 and PAK3.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Frédéric (Verified Customer) (02-18-2022)

Je suis satisfait du résultat car j’ai pu détecter par Western-blot une seule bande de forte intensité d’environ 69 kDa sur un lysat de plaquette humaine (voir figure ci-jointe).

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: lysat de plaquette humaine

PAK2 Antibody Western Blot validation (1/1000 dilution) in lysat de plaquette humaine (Cat no:19979-1-AP)