• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PAK2 Rekombinanter Antikörper

PAK2 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243124G9

Kat-Nr. : 85646-2-PBS

Synonyme

C t PAK2, EC:2.7.11.1, Gamma PAK, Gamma-PAK, p21 activated kinase 2

Filter:
  • Western Blot (WB) analysis of various lysates using PAK2 Recombinant monoclonal antibody (85646-2-RR)

    WB analysis using 85646-2-RR (same clone as 85646-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85646-2-RR (PAK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85646-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PAK2 Recombinant monoclonal antibody (85646-2-RR)

    IF Staining of HeLa using 85646-2-RR (same clone as 85646-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PAK2 antibody (85646-2-RR, Clone: 243124G9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85646-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85646-2-PBS bindet in WB, IF/ICC, Indirect ELISA PAK2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name p21 protein (Cdc42/Rac)-activated kinase 2
Berechnetes Molekulargewicht 58 kDa
Beobachtetes Molekulargewicht 54 kDa
GenBank-ZugangsnummerNM_002577
Gene symbol PAK2
Gene ID (NCBI) 5062
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAK2, also named as PAK65, PAKgamma, p58, PAK-2p27, PAK-2p24 and C-t-PAK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. PAK2 has several isoforms with the MW of 54-62 kDa and 41 kDa. The 62 kDa PAK2 is cleaved into a 34 kDa C terminal fragment and a 28 kDa N terminal fragment with a time course that parallels apoptotic death in Jurkat cells. (PMID:10200518). Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.