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PAK4 Polyklonaler Antikörper

PAK4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(23)

Kat-Nr. : 14685-1-AP

Synonyme

Serine/threonine-protein kinase PAK 4, PAK-4, PAK 4, p21-activated kinase 4, KIAA1142



Geprüfte Anwendungen

Erfolgreiche Detektion in WBCOLO 320-Zellen, HeLa-Zellen, PC-3-Zellen, Rattenhautgewebe
Erfolgreiche IPMauslebergewebe
Erfolgreiche Detektion in IHChumanes Lebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:250-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14685-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PAK4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PAK4 fusion protein Ag5981
Vollständiger Name p21 protein (Cdc42/Rac)-activated kinase 4
Berechnetes Molekulargewicht 64 kDa
Beobachtetes Molekulargewicht 64 kDa
GenBank-ZugangsnummerBC025282
Gene symbol PAK4
Gene ID (NCBI) 10298
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for PAK4 antibody 14685-1-APProtokoll herunterladen
IHC protocol for PAK4 antibody 14685-1-APProtokoll herunterladenl
IF protocol for PAK4 antibody 14685-1-APProtokoll herunterladen
IP protocol for PAK4 antibody 14685-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IF

Nat Commun

Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions.

Authors - Yohendran Baskaran
  • KD Validated
humanWB,IHC

Cancer Res

Targeting PAK4 Inhibits Ras-Mediated Signaling and Multiple Oncogenic Pathways in High-Risk Rhabdomyosarcoma.

Authors - Atreyi Dasgupta
humanIHC,WB

Mol Ther Nucleic Acids

ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway.

Authors - Lingjiao Meng
humanWB

Aging (Albany NY)

PAK4 suppresses TNF-induced release of endothelial microparticles in HUVECs cells.

Authors - Shouqin Zhang
  • KD Validated
mouseWB

Commun Biol

RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling

Authors - Haixia Zhu
humanWB

Mol Neurobiol

Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation

Authors - Ruicheng Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Juan (Verified Customer) (05-21-2024)

This primary antibody works pretty good!

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Cell culture
  • Primary Antibody Dilution: PAK4 Polyclonal antibody
  • Cell Tissue Type: human cells
FH

Emily (Verified Customer) (07-18-2022)

Good strong signal by western blot, intend to use for IF soon

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Breast cancer cell