PAK4 Monoklonaler Antikörper

PAK4 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2G6A3

Kat-Nr. : 68298-1-PBS

Synonyme

KIAA1142, p21 activated kinase 4, PAK 4, PAK4

---

• 

  WB analysis using 68298-1-Ig (same clone as 68298-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68298-1-Ig (PAK4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68298-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68298-1-Ig (same clone as 68298-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PAK4 antibody (68298-1-Ig, Clone: 2G6A3 ) at dilution of 1:3000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68298-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

68298-1-PBS bindet in WB, IF/ICC, Indirect ELISA PAK4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PAK4 fusion protein Ag6364
• Vollständiger Name: p21 protein (Cdc42/Rac)-activated kinase 4
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC025282
• Gene symbol: PAK4
• Gene ID (NCBI): 10298
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).