PAK4 Rekombinanter Antikörper

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• KD/KO Validated

PAK4 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240686D1

Kat-Nr. : 83761-5-RR

Synonyme

240686D1, EC:2.7.11.1, KIAA1142, p21-activated kinase 4, PAK 4

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  WB analysis using 83761-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83761-5-RR (PAK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 83761-5-RR

  WB result of PAK4 antibody (83761-5-RR; 1:3000; room temperature for 1.5 hours) with wild-type and sg-PAK4 transfected HAP1 cells.

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  FC experiment of MCF-7 using 83761-5-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PAK4 Recombinant antibody (83761-5-RR, Clone:240686D1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83761-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83761-5-RR against Human PAK4 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COLO 320-Zellen, MCF-7-Zellen, PC-3-Zellen, Raji-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83761-5-RR bindet in WB, FC (Intra), ELISA PAK4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PAK4 fusion protein Ag5981
• Vollständiger Name: p21 protein (Cdc42/Rac)-activated kinase 4
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC025282
• Gene symbol: PAK4
• Gene ID (NCBI): 10298
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).