PAK6 Polyklonaler Antikörper

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• KD/KO Validated

PAK6 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13539-1-PBS

Synonyme

PAK5, PAK 6, PAK 5, p21-activated kinase 6, p21 activated kinase 6

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  WB analysis using 13539-1-AP (same clone as 13539-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13539-1-AP (PAK6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 13539-1-AP (same clone as 13539-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 13539-1-AP (PAK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 13539-1-AP (same clone as 13539-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13539-1-AP (PAK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 13539-1-AP (same clone as 13539-1-PBS)

  IP result of anti-PAK6 (IP:13539-1-AP, 4ug; Detection:13539-1-AP 1:300) with mouse testis tissue lysate 4000 ug. This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 13539-1-AP (same clone as 13539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13539-1-AP (PAK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 13539-1-AP (same clone as 13539-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13539-1-AP (PAK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 13539-1-AP (same clone as 13539-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PAK6 antibody (13539-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13539-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13539-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PAK6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAK6 fusion protein Ag4467
• Vollständiger Name: p21 protein (Cdc42/Rac)-activated kinase 6
• Berechnetes Molekulargewicht: 681 aa, 75 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC035596
• Gene symbol: PAK6
• Gene ID (NCBI): 56924
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661). The immunogen of this antibody is the C-terminal of PAK6. This antibody has weak cross-reaction with other PAK members.