PAK7 Rekombinanter Antikörper

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PAK7 Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230201A10

Kat-Nr. : 82935-1-PBS

Synonyme

PAK4/6/7, 230201A10, EC:2.7.11.1, KIAA1264, p21-activated kinase 5

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  WB analysis using 82935-1-RR (same clone as 82935-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82935-1-RR (PAK7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82935-1-RR (same clone as 82935-1-PBS)

  WB result of PAK7 antibody (82935-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK7 transfected HeLa cells. This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 82935-1-RR (same clone as 82935-1-PBS)

  (blue)1x10^6 K-562 cells were intracellularly stained with 0.25 ug Anti-Human PAK7 (82935-1-RR, Clone:230201A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82935-1-RR (same clone as 82935-1-PBS)

  (blue)1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human PAK7 (82935-1-RR, Clone:230201A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82935-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82935-1-PBS bindet in WB, FC (Intra), Indirect ELISA PAK7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PAK7 fusion protein Ag29190
• Vollständiger Name: p21 protein (Cdc42/Rac)-activated kinase 7
• Berechnetes Molekulargewicht: 719 aa, 81 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC024179
• Gene symbol: PAK7
• Gene ID (NCBI): 57144
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAK7, also known as PAK5, is a newly found but scarcely researched type II PAKs (PAK4, 5, 6). PAK7 was originally found in the brain, which promotes filopodia formation in nerve cells. Recently, studies have found that the expression level of PAK7 significantly increased in colorectal cancer, ovarian cancer, gastric cancer, neuroglioma, hepatocellular carcinoma, pancreatic cancer, osteosarcoma, and breast cancer. PAK7 plays an important role in tumorigenesis, invasion, and metastasis, mainly related to its mechanism of regulating cytoskeleton, inhibiting apoptosis, and promoting proliferation. The calculated molecular weight of PAK7 is 80 kDa (PMID: 29805709, PMID: 34165002).