PALMD Polyklonaler Antikörper

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PALMD Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16531-1-PBS

Synonyme

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  WB analysis of HeLa using 16531-1-AP (same clone as 16531-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16531-1-AP (same clone as 16531-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 16531-1-AP (same clone as 16531-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse heart using 16531-1-AP (same clone as 16531-1-PBS)

  IP result of anti-PALMD (IP:16531-1-AP, 5ug; Detection:16531-1-AP 1:500) with mouse heart tissue lysate 6000ug. This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

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  IP experiment using 16531-1-AP (same clone as 16531-1-PBS)

  IP result of PALMD antibody (16531-1-AP for Ip and Detection) with mouse heart tissue lysate. This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16531-1-AP (same clone as 16531-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16531-1-AP (PALMD antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16531-1-AP (same clone as 16531-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16531-1-AP (PALMD antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16531-1-PBS bindet in WB, IHC, IP, Indirect ELISA PALMD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PALMD fusion protein Ag9796
• Vollständiger Name: palmdelphin
• Berechnetes Molekulargewicht: 551 aa, 63 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC012570
• Gene symbol: PALMD
• Gene ID (NCBI): 54873
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Palmdelphin, encoded by PALMD gene, is a newly identified cytosolic isoform of paralemmin-1, a lipid raft-associated protein implicated in cell shape control. Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Previous finding suggest that palmdelphin may peripherally associate with endomembranes or cytoskeleton-linked structures. Palmdelphin appears to be 80 kDa in Western blotting, larger than the predicated of 63 kDa, and this may be due to the acidic nature of this protein or its posttranslational modification.