PAN2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PAN2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 16427-1-AP

Synonyme

EC:3.1.13.4, hPAN2, Inactive ubiquitin carboxyl-terminal hydrolase 52, KIAA0710, PAB1P-dependent poly(A)-specific ribonuclease

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  WB analysis of NIH/3T3 using 16427-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16427-1-AP (PAN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16427-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16427-1-AP (PAN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse heart using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16427-1-AP (PAN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16427-1-AP (PAN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 16427-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PAN2 antibody (16427-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Hirngewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Plazenta-Gewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

16427-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PAN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAN2 fusion protein Ag9670
• Vollständiger Name: PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 1198 aa, 135 kDa
• Beobachtetes Molekulargewicht: 135 kDa
• GenBank-Zugangsnummer: BC024043
• Gene symbol: PAN2
• Gene ID (NCBI): 9924
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC,IF	Nat Commun; USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization.; Authors - Shangda Yang ; KD Validated
	WB	Nat Struct Mol Biol; An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to mediate mRNA deadenylation and decay.; Authors - Stefanie Jonas
human	IF	Neurobiol Dis; Distinct Poly(A) nucleases have differential impact on sut-2 dependent tauopathy phenotypes.; Authors - Rebecca L Kow
mouse	WB	Biol Reprod; Mobilization of Dormant Cnot7 mRNA Promotes Deadenylation of Maternal Transcripts During Mouse Oocyte Maturation.; Authors - Jun Ma
human	WB,IP	PLoS One; Anti-PABPC1 Co-Immunoprecipitation for Examining the miRNAs Directly Targeting the 3'-UTR of EED mRNA.; Authors - Yi Hu
mouse	WB	Cells; Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).; Authors - Tian-Li Duan