PAN3 Polyklonaler Antikörper

PAN3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25655-1-PBS

Synonyme

hPan3, PAB1P-dependent poly(A)-specific ribonuclease, PAN deadenylation complex subunit 3, PAN2-PAN3 deadenylation complex subunit PAN3, Poly(A)-nuclease deadenylation complex subunit 3

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  WB analysis using 25655-1-AP (same clone as 25655-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25655-1-AP (PAN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25655-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 25655-1-AP (same clone as 25655-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25655-1-AP (PAN3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25655-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25655-1-PBS bindet in WB, IHC, Indirect ELISA PAN3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAN3 fusion protein Ag22559
• Vollständiger Name: PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 887 aa, 96 kDa
• Beobachtetes Molekulargewicht: 90-100kDa; 65-68 kDa
• GenBank-Zugangsnummer: BC128180
• Gene symbol: PAN3
• Gene ID (NCBI): 255967
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Shortening of the poly(A) tail in mRNA, called deadenylation, is the first rate-limiting step in mRNA turnover. PAN3 is the regulatory subunit of a cytoplasmic poly(A) nuclease complex that links the catalytic PAN2 subunit to the poly(A)-binding protein PABP, which stimulates PAN2-dependent deadenylation activity (PMID: 14583602). PAN3 has four isoforms, isoform1: 95 kDa; isoform2: 64 kDa, isoform3: 89 kDa and isoform4: 72 kDa. This antibody recognizes isoform 1/2/3.