PANK2 Polyklonaler Antikörper

PANK2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11001-1-PBS

Synonyme

Pantothenate kinase 2, mitochondrial intermediate form, mPanK2, iPanK2, hPanK2, EC:2.7.1.33

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  WB analysis using 11001-1-AP (same clone as 11001-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11001-1-AP (same clone as 11001-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11001-1-AP (PANK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11001-1-AP (same clone as 11001-1-PBS)

  IP result of anti-PANK2 (IP:11001-1-AP, 3ug; Detection:11001-1-AP 1:800) with mouse brain tissue lysate 9500ug. This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 11001-1-AP (same clone as 11001-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11001-1-AP (PANK2 antibody) at dilution of 1:10 (under 10x lens). This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11001-1-AP (same clone as 11001-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 11001-1-AP (PANK2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11001-1-AP (same clone as 11001-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 11001-1-AP (PANK2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11001-1-AP (same clone as 11001-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PANK2 antibody (11001-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 11001-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11001-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PANK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PANK2 fusion protein Ag1413
• Vollständiger Name: pantothenate kinase 2
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 63-70 kDa
• GenBank-Zugangsnummer: BC009421
• Gene symbol: PANK2
• Gene ID (NCBI): 80025
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pantothenate kinase 2 (PANK2) is an essential regulatory enzyme in coenzyme A biosynthesis. PANK2 mutations cause pantothenate kinase-associated neurodegeneration (PKAN), which leads to pigmentary retinopathy, progressive dystonia and other abnormalities(PMID:20603201). PanK2 protein is localized to mitochondria of neurons in human brain, distinguishing it from other pantothenate kinases that do not possess mitochondrial-targeting sequences(PMID:15659606 ). It has 4 isoforms produced by alternative splicing and alternative initiation.