PAPOLG Polyklonaler Antikörper

PAPOLG Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24284-1-PBS

Synonyme

---

• 

  WB analysis of HeLa using 24284-1-AP (same clone as 24284-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 24284-1-AP (same clone as 24284-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 24284-1-AP (same clone as 24284-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24284-1-AP (PAPOLG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 24284-1-AP (same clone as 24284-1-PBS)

  IP result of anti-PAPOLG (IP:24284-1-AP, 4ug; Detection:24284-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 24284-1-AP (same clone as 24284-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 24284-1-AP (same clone as 24284-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24284-1-AP (PAPOLG Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 24284-1-AP (same clone as 24284-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PAPOLG antibody (24284-1-AP) at dilution of 1:1600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24284-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

24284-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PAPOLG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAPOLG fusion protein Ag16208
• Vollständiger Name: poly(A) polymerase gamma
• Berechnetes Molekulargewicht: 736 aa, 83 kDa
• Beobachtetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC111701
• Gene symbol: PAPOLG
• Gene ID (NCBI): 64895
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAPOLG, also named as Neo-poly(A) polymerase or Polynucleotide adenylyltransferase gamma, is a 736 amino acid protein, which belongs to the poly(A) polymerase family. PAPOLG is expressed predominantly in testis, and weakly in other tissues. PAPOLG is responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.