PAPPA Polyklonaler Antikörper

PAPPA Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18095-1-PBS

Synonyme

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  WB analysis using 18095-1-AP (same clone as 18095-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18095-1-AP (PAPPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18095-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 18095-1-AP (same clone as 18095-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18095-1-AP (PAPPA antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18095-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 18095-1-AP (same clone as 18095-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18095-1-AP (PAPPA antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18095-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18095-1-PBS bindet in WB, IHC, Indirect ELISA PAPPA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAPPA fusion protein Ag11863
• Vollständiger Name: pregnancy-associated plasma protein A, pappalysin 1
• Berechnetes Molekulargewicht: 1627 aa, 181 kDa
• Beobachtetes Molekulargewicht: 180-220 kDa
• GenBank-Zugangsnummer: BC078657
• Gene symbol: PAPPA
• Gene ID (NCBI): 5069
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pregnancy-associated plasma protein A (PAPPA) is a metalloproteinase secreted by human placenta, and it can interact with IGF-binding proteins (IGFBPs) to regulate the proteolysis and the bioavailability of insulin-like growth factor (IGF). Circulating PAPPA represents a consolidated biomarker in combined first-trimester screening tests, allowing an early prenatal diagnosis of chromosomal abnormalities, preeclampsia, and intrauterine growth restriction risk. PAPPA is highly expressed in placenta and pregnancy serum (PMID: 34974815, 34974815).