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PAPPA Monoklonaler Antikörper

PAPPA Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G5A7

Kat-Nr. : 66962-1-PBS

Synonyme

1G5A7, ASBABP2, DIPLA1, EC:3.4.24.79, IGFBP 4ase

Filter:
  • Western Blot (WB) analysis of human placenta tissue using PAPPA Monoclonal antibody (66962-1-Ig)

    WB analysis of human placenta using 66962-1-Ig (same clone as 66962-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66962-1-Ig (PAPPA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66962-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using PAPPA Monoclonal antibody (66962-1-Ig)

    WB analysis of MCF-7 using 66962-1-Ig (same clone as 66962-1-PBS)

    WB result of PAPPA antibody (66962-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAPPA transfected MCF-7 cells. This data was developed using the same antibody clone with 66962-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PAPPA Monoclonal antibody (66962-1-Ig)

    IHC staining of human placenta using 66962-1-Ig (same clone as 66962-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66962-1-Ig (PAPPA antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66962-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PAPPA Monoclonal antibody (66962-1-Ig)

    IHC staining of human placenta using 66962-1-Ig (same clone as 66962-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66962-1-Ig (PAPPA antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66962-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66962-1-PBS bindet in WB, IHC, ELISA PAPPA und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen PAPPA fusion protein Ag11863
Vollständiger Name pregnancy-associated plasma protein A, pappalysin 1
Berechnetes Molekulargewicht 1627 aa, 181 kDa
Beobachtetes Molekulargewicht 180-220 kDa
GenBank-ZugangsnummerBC078657
Gene symbol PAPPA
Gene ID (NCBI) 5069
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pregnancy-associated plasma protein A (PAPPA) is a metalloproteinase secreted by human placenta, and it can interact with IGF-binding proteins (IGFBPs) to regulate the proteolysis and the bioavailability of insulin-like growth factor (IGF). Circulating PAPPA represents a consolidated biomarker in combined first-trimester screening tests, allowing an early prenatal diagnosis of chromosomal abnormalities, preeclampsia, and intrauterine growth restriction risk. PAPPA is highly expressed in placenta and pregnancy serum (PMID: 34974815, 34974815).