PARD6B Polyklonaler Antikörper

PARD6B Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 13996-1-AP

Synonyme

PAR-6 beta, PAR6B, PAR-6B, Partitioning defective 6 homolog beta



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, LNCaP-Zellen, PC-3-Zellen
Erfolgreiche IPHEK-293-Zellen
Erfolgreiche Detektion in IHCMausnierengewebe, humanes Pankreasgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13996-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PARD6B und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PARD6B fusion protein Ag5083
Vollständiger Name par-6 partitioning defective 6 homolog beta (C. elegans)
Berechnetes Molekulargewicht 41 kDa
Beobachtetes Molekulargewicht 50-53 kDa
GenBank-ZugangsnummerBC060847
Gene symbol PARD6B
Gene ID (NCBI) 84612
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARD6B (also named PAR6B) is a member of the PAR6 family. PARD6B is known to play a key role in mammary epithelial cell biology. Several studies have been reported which indicate deregulated PARD6B signaling contributes to malignant epithelial cell phenotypes due predominantly to disrupted polymerization and maintenance of tight junctions (PMID: 22957302). The approximately 4.8-kb long PARD6B mRNA was predominantly detected in both adult and fetal kidneys, while much weaker but significant signals were observed in the placenta, lung, and liver (PMID: 11260256). This antibody detects PARD6B with an apparent molecular weight of 50-53 kDa as has been demonstrated by several researches (PMID: 22496418; 25662318).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for PARD6B antibody 13996-1-APProtokoll herunterladen
IHC protocol for PARD6B antibody 13996-1-APProtokoll herunterladenl
IF protocol for PARD6B antibody 13996-1-APProtokoll herunterladen
IP protocol for PARD6B antibody 13996-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratIF

iScience

Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration

Authors - Haibin Tian
mouseWB

PLoS One

PKCζ and JNK signaling regulate radiation-induced compensatory proliferation in parotid salivary glands.

Authors - Wen Yu Wong
WB

Theriogenology

Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway.

Authors - Xue Wang
humanWB,IHC

Cancer Lett

Oncogenic functions and therapeutic potentials of targeted inhibition of SMARCAL1 in small cell lung cancer

Authors - Bei-Bei Sun

Cancer Gene Ther

PRAD6A promotes lung adenocarcinoma cell proliferation and invasion through Serpina3

Authors - Lanlin Hu
humanWB

J Agric Food Chem

Discovery and Functional Characterization of a Recombinant Fragment of Human Collagen Type XVII

Authors - Lianhua Piao