PARD6B Polyklonaler Antikörper

PARD6B Polyklonal Antikörper für FC, IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13996-1-AP

Synonyme

PAR 6 beta, PAR 6B, PAR6B, PARD6B

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 13996-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13996-1-AP (PARD6B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13996-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13996-1-AP (PARD6B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13996-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13996-1-AP (PARD6B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13996-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13996-1-AP (PARD6B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 13996-1-AP

  IP Result of anti-PARD6B (IP:13996-1-AP, 3ug; Detection:13996-1-AP 1:200) with HEK-293 cells lysate 6000ug.

• 

  IHC staining of mouse kidney using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13996-1-AP (PARD6B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13996-1-AP (PARD6B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13996-1-AP (PARD6B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 13996-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 13996-1-AP (PARD6B antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 13996-1-AP

  Immunofluorescent analysis of HepG2 cells, using PARD6B antibody 13996-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of MCF-7 using 13996-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PARD6B antibody 13996-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 13996-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug PARD6B antibody (13996-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, humanes Plazenta-Gewebe, PC-3-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

13996-1-AP bindet in WB, IP, IHC, IF, FC, ELISA PARD6B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PARD6B fusion protein Ag5083
• Vollständiger Name: par-6 partitioning defective 6 homolog beta (C. elegans)
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 50-53 kDa
• GenBank-Zugangsnummer: BC060847
• Gene symbol: PARD6B
• Gene ID (NCBI): 84612
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARD6B (also named as PAR6B) is a member of the PAR6 family. PARD6B is known to play a key role in mammary epithelial cell biology. Several studies have been reported which indicate deregulated PARD6B signaling contributes to malignant epithelial cell phenotypes due predominantly to disrupted polymerization and maintenance of tight junctions (PMID: 22957302). The approximately 4.8-kb long PARD6B mRNA was predominantly detected in both adult and foetal kidneys, while much weaker but significant signals were observed in the placenta, lung and liver (PMID: 11260256). This antibody detects PARD6B with an apparent molecular weight of 50-53 kDa as has been demonstrated by several researches (PMID: 22496418; 25662318).

Publikationen

Species	Application	Title
rat	IF	iScience; Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration; Authors - Haibin Tian
mouse	WB	PLoS One; PKCζ and JNK signaling regulate radiation-induced compensatory proliferation in parotid salivary glands.; Authors - Wen Yu Wong
	WB	Theriogenology; Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway.; Authors - Xue Wang

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