PARG Monoklonaler Antikörper

PARG Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H10G10

Kat-Nr. : 67946-1-PBS

Synonyme

PARG, PARG99

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  WB analysis using 67946-1-Ig (same clone as 67946-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67946-1-Ig (PARG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67946-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67946-1-Ig (same clone as 67946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67946-1-Ig (PARG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67946-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67946-1-Ig (same clone as 67946-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67946-1-Ig (PARG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67946-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67946-1-Ig (same clone as 67946-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PARG antibody (67946-1-Ig, Clone: 1H10G10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67946-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67946-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PARG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PARG fusion protein Ag27288
• Vollständiger Name: poly (ADP-ribose) glycohydrolase
• Berechnetes Molekulargewicht: 111 kDa
• Beobachtetes Molekulargewicht: 111 kDa
• GenBank-Zugangsnummer: BC052966
• Gene symbol: PARG
• Gene ID (NCBI): 8505
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.