PARK7/DJ-1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PARK7/DJ-1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11681-1-PBS

Synonyme

PARK7, PARK7,DJ-1, PARK 7, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124

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  WB analysis of HeLa using 11681-1-AP (same clone as 11681-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11681-1-AP (same clone as 11681-1-PBS)

  WB result of PARK7/DJ-1 antibody (11681-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PARK7/DJ-1 transfected HEK-293 cells. This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11681-1-AP (same clone as 11681-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 11681-1-AP (same clone as 11681-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11681-1-AP (same clone as 11681-1-PBS)

  IP result of anti-PARK7/DJ-1 (IP:11681-1-AP, 4ug; Detection:11681-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7/DJ-1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11681-1-AP (PARK7/DJ-1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11681-1-AP (PARK7/DJ-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7/DJ-1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11681-1-AP (PARK7/DJ-1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11681-1-AP (same clone as 11681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11681-1-AP (PARK7,DJ-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 11681-1-AP (same clone as 11681-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PARK7,DJ-1 antibody (11681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11681-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11681-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PARK7/DJ-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PARK7/DJ-1 fusion protein Ag2287
• Vollständiger Name: Parkinson disease (autosomal recessive, early onset) 7
• Berechnetes Molekulargewicht: 189 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa, 25 kDa
• GenBank-Zugangsnummer: BC008188
• Gene symbol: PARK7
• Gene ID (NCBI): 11315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).