PARK7/DJ-1 Monoklonaler Antikörper

PARK7/DJ-1 Monoklonal Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, Huhn, human, Kaninchen, Maus, Ratte

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4G4E7

Kat-Nr. : 68915-6-PBS

Synonyme

PARK7, PARK7,DJ-1, 4G4E7, DJ 1, DJ1

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  WB analysis using 68915-6-Ig (same clone as 68915-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68915-6-Ig (PARK7/DJ-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

• 

  WB analysis using 68915-6-Ig (same clone as 68915-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68915-6-Ig (PARK7/DJ-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

• 

  WB analysis using 68915-6-Ig (same clone as 68915-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68915-6-Ig (PARK7/DJ-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

• 

  FC experiment of SKOV-3 using 68915-6-Ig (same clone as 68915-6-PBS)

  1x10^6 SKOV-3 cells were intracellularly stained with 0.2 μg PARK7/DJ-1 Monoclonal antibody (68915-6-Ig, Clone: 4G4E7, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68915-6-Ig (same clone as 68915-6-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.2 μg PARK7/DJ-1 Monoclonal antibody (68915-6-Ig, Clone: 4G4E7, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 68915-6-Ig (same clone as 68915-6-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.2 μg PARK7/DJ-1 Monoclonal antibody (68915-6-Ig, Clone: 4G4E7, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

• 

  FC experiment of Raji using 68915-6-Ig (same clone as 68915-6-PBS)

  1x10^6 Raji cells were intracellularly stained with 0.2 μg PARK7/DJ-1 Monoclonal antibody (68915-6-Ig, Clone: 4G4E7, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 68915-6-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68915-6-PBS bindet in WB, FC (Intra), Indirect ELISA PARK7/DJ-1 und zeigt Reaktivität mit Hausschwein, Huhn, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Huhn, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PARK7/DJ-1 fusion protein Ag28526
• Vollständiger Name: Parkinson disease (autosomal recessive, early onset) 7
• Berechnetes Molekulargewicht: 189 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC008188
• Gene symbol: PARK7
• Gene ID (NCBI): 11315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).