PARK7/DJ-1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

PARK7/DJ-1 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

230124B7

Kat-Nr. : 82913-2-RR

Synonyme

PARK7, PARK7,DJ-1, Maillard deglycase, EC:3.1.2.-, EC:3.5.1.-, EC:3.5.1.124, DJ-1

---

• 

  WB analysis of HEK-293 using 82913-2-RR

  WB result of PARK7/DJ-1 antibody (82913-2-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PARK7/DJ-1 transfected HEK-293 cells.

• 

  WB analysis using 82913-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82913-2-RR (PARK7/DJ-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 82913-2-RR

  IP result of anti-PARK7/DJ-1 (IP:82913-2-RR, 4ug; Detection:82913-2-RR 1:2000) with HeLa cells lysate 1280 ug.

• 

  IF Staining of SH-SY5Y using 82913-2-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PARK7/DJ-1 antibody (82913-2-RR, Clone: 230124B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HeLa using 82913-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PARK7/DJ-1 Recombinant antibody (82913-2-RR, Clone:230124B7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 82913-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82913-2-RR against Human PARK7/DJ-1 were performed. The affinity constant is 7.89 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, BxPC-3-Zellen, HEK-293-Zellen, Jurkat-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82913-2-RR bindet in WB, IF/ICC, FC (Intra), IP, ELISA PARK7/DJ-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PARK7/DJ-1 fusion protein Ag2287
• Vollständiger Name: Parkinson disease (autosomal recessive, early onset) 7
• Berechnetes Molekulargewicht: 189 aa, 20 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC008188
• Gene symbol: PARK7
• Gene ID (NCBI): 11315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARK7, also named as DJ1, belongs to the peptidase C56 family. It protects cells against oxidative stress and cell death. PARK7 plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. It eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. PARK7 has cell-growth promoting activity and transforming activity. It may function as a redox-sensitive chaperone. It's precursor undergoes a cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. The amino acid replace at 166 (L → P) reduces PARK7 protein stability and leads to increased degradation. The predicted MW of this protein is 20 kDa, An additional 25 kDa band can be observed due to modification (PMID: 31767755).