PARP1 Monoklonaler Antikörper

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• KD/KO Validated

PARP1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

1D7D4

Kat-Nr. : 66520-1-PBS

Synonyme

1D7D4, ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT, ADPRT 1, ADPRT1

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  WB analysis of Jurkat using 66520-1-Ig (same clone as 66520-1-PBS)

  Jurkat cells (20 μg/lane) treated with staurosporine were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66520-1-Ig (same clone as 66520-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  WB analysis using 66520-1-Ig (same clone as 66520-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66520-1-Ig (same clone as 66520-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66520-1-Ig (same clone as 66520-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66520-1-Ig (same clone as 66520-1-PBS)

  HeLa cells (20 μg) were subjected to SDS PAGE followed by western blot with 66520-1-Ig (PARP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  Sample test of MP50985-1

  Cytometric bead array in cell lysate using MP50985-1, Cleaved PARP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66520-1-PBS. Detection antibody: 60555-2-PBS. Cell lysate: Non-treated Jurkat and Staurosporine treated Jurkat (10μg/well). Non-related target Tubulin-beta Recombinant Matched Antibody Pair (MP00550-1) was served as control.

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  Sample test of MP50985-2

  Cytometric bead array in cell lysate using MP50985-2, Cleaved PARP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60555-1-PBS. Detection antibody: 66520-1-PBS. Cell lysate: Non-treated Jurkat and Staurosporine treated Jurkat (10μg/well). Non-related target Tubulin-beta Recombinant Matched Antibody Pair (MP00550-1) was served as control.

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  Sample test of MP50985-3

  Cytometric bead array in cell lysate using MP50985-3, Human only PARP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60555-3-PBS. Detection antibody: 66520-1-PBS. Cell lysate: Non-treated Jurkat and Staurosporine treated Jurkat (10μg/well). Non-related target Tubulin-beta Recombinant Matched Antibody Pair (MP00550-1) was served as control.

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  IP experiment of K-562 using 66520-1-Ig (same clone as 66520-1-PBS)

  IP result of anti-PARP1 (IP:66520-1-Ig, 5ug; Detection:66520-1-Ig 1:10000) with K-562 cells lysate 2760 ug. This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 66520-1-Ig (same clone as 66520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66520-1-Ig (same clone as 66520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66520-1-Ig (same clone as 66520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66520-1-Ig (same clone as 66520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 66520-1-Ig (same clone as 66520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66520-1-Ig (same clone as 66520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66520-1-Ig (same clone as 66520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66520-1-Ig (PARP1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 66520-1-Ig (same clone as 66520-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PARP1 antibody (66520-1-Ig, Clone: 1D7D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66520-1-Ig (same clone as 66520-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PARP1 (66520-1-Ig, Clone:1D7D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 66520-1-Ig (same clone as 66520-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human PARP1 (66520-1-Ig, Clone:1D7D4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66520-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66520-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA, Sample test PARP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PARP1 fusion protein Ag19173
• Vollständiger Name: poly (ADP-ribose) polymerase 1
• Berechnetes Molekulargewicht: 1014 aa, 113 kDa
• Beobachtetes Molekulargewicht: 113-116 kDa, 85-89 kDa
• GenBank-Zugangsnummer: BC037545
• Gene symbol: PARP1
• Gene ID (NCBI): 142
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89-kDa. This antibody was generated against the N-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.