PARP1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

PARP1 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

3N19

Kat-Nr. : 80174-1-PBS

Synonyme

3N19, ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT, ADPRT 1, ADPRT1

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  WB analysis using 80174-1-RR (same clone as 80174-1-PBS)

  Jurkat cells treated with Staurosporin were subjected to SDS PAGE followed by western blot with 80174-1-RR (PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 80174-1-RR (same clone as 80174-1-PBS)

  WB result of PARP1 antibody (80174-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PARP1 transfected HEK-293 cells. This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80174-1-RR (same clone as 80174-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80174-1-RR (PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80174-1-RR (same clone as 80174-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80174-1-RR (PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 80174-1-RR (same clone as 80174-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80174-1-RR (PARP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 80174-1-RR (same clone as 80174-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80174-1-RR (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 80174-1-RR (same clone as 80174-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80174-1-RR (PARP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 80174-1-RR (same clone as 80174-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 80174-1-RR (same clone as 80174-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 80174-1-RR (same clone as 80174-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 80174-1-RR (same clone as 80174-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using PARP1 antibody (80174-1-RR, Clone: 3N19 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

• 

  ChIP experiment of HeLa using 80174-1-RR (same clone as 80174-1-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of PARP1 (80174-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 80174-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80174-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80174-1-RR against Human PARP1 were performed. The affinity constant is 0.639 nM.

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Geprüfte Anwendungen

Produktinformation

80174-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA, ChIP-qPCR PARP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PARP1 fusion protein Ag4193
• Vollständiger Name: poly (ADP-ribose) polymerase 1
• Berechnetes Molekulargewicht: 1014 aa, 113 kDa
• Beobachtetes Molekulargewicht: 113-116, 89 kDa
• GenBank-Zugangsnummer: BC037545
• Gene symbol: PARP1
• Gene ID (NCBI): 142
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.