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PARP14 Rekombinanter Antikörper

PARP14 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241078C11

Kat-Nr. : 84039-6-RR

Synonyme

PARP 14, KIAA1268, BAL2, 241078C11

Filter:
  • Western Blot (WB) analysis of various lysates using PARP14 Recombinant antibody (84039-6-RR)

    WB analysis using 84039-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84039-6-RR (PARP14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using PARP14 Recombinant antibody (84039-6-RR)

    IHC staining of mouse colon using 84039-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84039-6-RR (PARP14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84039-6-RR

    Affinity and Kinetic Characterization of 84039-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84039-6-RR against Human PARP14 were performed. The affinity constant is 0.685 nM.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, U2OS-Zellen
Erfolgreiche Detektion in IHCMaus-Kolongewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

84039-6-RR bindet in WB, IHC, ELISA PARP14 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PARP14 fusion protein Ag35007
Vollständiger Name poly (ADP-ribose) polymerase family, member 14
Berechnetes Molekulargewicht203kd
Beobachtetes Molekulargewicht200 kDa
GenBank-ZugangsnummerNM_017554
Gene symbol PARP14
Gene ID (NCBI) 54625
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARP14 (poly (ADP-ribose) polymerase family, member 14) is a PARP family member that has roles in DNA repair, B cell regulation, and focal adhesion (PMID: 25753673). The largest of all the human PARPs is PARP14. PARP14 has been reported to regulate several different pathways involved in immunity, inflammation, and genome stability (PMID: 37703374).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for PARP14 antibody 84039-6-RRProtokoll herunterladen
IHC protocol for PARP14 antibody 84039-6-RRProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Lisa (Verified Customer) (07-24-2025)

Produces clear signals with minimal background and can be reused multiple times without performance loss. Works very well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000