PARP4 Polyklonaler Antikörper

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PARP4 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19775-1-PBS

Synonyme

ADP-ribosyltransferase diphtheria toxin-like 4, ADPRTL1, ARTD4, EC:2.4.2.-, p193

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  WB analysis using 19775-1-AP (same clone as 19775-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 19775-1-AP (PARP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19775-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 19775-1-AP (same clone as 19775-1-PBS)

  WB result of PARP4 antibody (19775-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PARP4 transfected A549 cells. This data was developed using the same antibody clone with 19775-1-PBS in a different storage buffer formulation.

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  WB analysis using 19775-1-AP (same clone as 19775-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 19775-1-AP (PARP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19775-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 19775-1-AP (same clone as 19775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19775-1-AP (PARP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19775-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 19775-1-AP (same clone as 19775-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19775-1-AP (PARP4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19775-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 19775-1-AP (same clone as 19775-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed 48孔 HepG2 cells using PARP4 antibody (19775-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19775-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19775-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PARP4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: poly (ADP-ribose) polymerase family, member 4
• Berechnetes Molekulargewicht: 193 kDa
• Beobachtetes Molekulargewicht: 193 kDa
• GenBank-Zugangsnummer: NM_006437
• Gene symbol: PARP4
• Gene ID (NCBI): 143
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PARP4, also named as ADPRTL1, KIAA0177, PARPL, VPARP and PH5P, catalyzes the reaction: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.