PASK Polyklonaler Antikörper

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PASK Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14396-1-PBS

Synonyme

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  WB analysis of HEK-293 using 14396-1-AP (same clone as 14396-1-PBS)

  WB result of PASK antibody (14396-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-PASK transfected HEK-293 cells. This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14396-1-AP (same clone as 14396-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 14396-1-AP (same clone as 14396-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 14396-1-AP (PASK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 14396-1-AP (same clone as 14396-1-PBS)

  IP result of anti-PASK (IP:14396-1-AP, 4ug; Detection:14396-1-AP 1:300) with K-562 cells lysate 4000 ug. This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 14396-1-AP (same clone as 14396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14396-1-AP (PASK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 14396-1-AP (same clone as 14396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14396-1-AP (PASK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate hyperplasia using 14396-1-AP (same clone as 14396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate hyperplasia using 14396-1-AP (same clone as 14396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 14396-1-AP (same clone as 14396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 14396-1-AP (same clone as 14396-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14396-1-AP (PASK Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14396-1-AP (same clone as 14396-1-PBS)

  Immunofluorescent analysis of HeLa cells using 14396-1-AP (PASK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14396-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14396-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PASK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PASK fusion protein Ag5893
• Vollständiger Name: PAS domain containing serine/threonine kinase
• Berechnetes Molekulargewicht: 143 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: BC063585
• Gene symbol: PASK
• Gene ID (NCBI): 23178
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAS (Per-Arnt-Sim) domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. PAS domain-containing serine/threonine-protein kinase (PASK, also termed PAS-kinase or PASKIN) is an evolutionarily conserved nutrient responsive protein kinase. PASK links energy flux and protein synthesis in yeast and regulates glycogen synthesis in mammals. It has also been implicated in glucose-stimulated INS production in pancreatic beta-cells.