PAX3 Polyklonaler Antikörper

PAX3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 51036-2-PBS

Synonyme

CDHS, paired box 3, Paired box protein Pax 3, Paired box protein Pax-3, PAX-3

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  WB analysis using 51036-2-AP (same clone as 51036-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse skin using 51036-2-AP (same clone as 51036-2-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 51036-2-AP (same clone as 51036-2-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 51036-2-AP (same clone as 51036-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 51036-2-AP (same clone as 51036-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 51036-2-AP (PAX3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 51036-2-AP (same clone as 51036-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 51036-2-AP (PAX3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

51036-2-PBS bindet in WB, IHC, Indirect ELISA PAX3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAX3 fusion protein Ag0477
• Vollständiger Name: paired box 3
• Berechnetes Molekulargewicht: 120 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC114363
• Gene symbol: PAX3
• Gene ID (NCBI): 5077
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAX3, a transcription factor and multifunctional regulatory protein, is normally expressed during embryonic development. In the nervous system, PAX3 is involved in neural tube closure, neural crest development, and peripheral neuron differentiation. In the present study, PAX3 was reported as a novel regulator of GFAP transcription, and the overexpression and suppression of PAX3 could inhibit and promote NSC differentiation, respectively. In muscle development, PAX3 ensures the survival of myogenic progenitor cells with Pax3-expressing progenitors giving rise to both skeletal and smooth muscle cells. PAX3 also has a well-established role in the development of melanocytes during embryogenesis, and has recently been characterized as a molecular switch in the mature melanocyte. Mutations in PAX3 can cause Waardenburg syndrome.