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PAX6 Polyklonaler Antikörper

PAX6 Polyklonal Antikörper für WB, IHC, IF, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(75)

Kat-Nr. : 12323-1-AP

Synonyme

paired box 6, Paired box protein Pax 6, Paired box protein Pax-6, PAX 6, Pax-6



Geprüfte Anwendungen

Erfolgreiche Detektion in WBY79-Zellen, Mausembryogewebe, Rattenhirngewebe
Erfolgreiche Detektion in IHCRatten-Augengewebe, humanes Retinoblastom-Gewebe, Maushirngewebe, Maus-Cerebellum-Gewebe, Mausembryogewebe, Maus-Augengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMaus-Augengewebe, Ratten-Augengewebe
Erfolgreiche Detektion in IFRetinal organoids, iPS-Zellen
Erfolgreiche Detektion in FC (Intra)SH-SY5Y-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
Immunhistochemie (IHC)IHC : 1:1000-1:4000
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
Immunfluoreszenz (IF)IF : 1:10-1:100
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

12323-1-AP bindet in WB, IHC, IF, IF-P, FC (Intra), ELISA PAX6 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, Maus, Ratte, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PAX6 fusion protein Ag2984
Vollständiger Name paired box 6
Berechnetes Molekulargewicht 47 kDa
Beobachtetes Molekulargewicht 47 kDa
GenBank-ZugangsnummerBC011953
Gene symbol PAX6
Gene ID (NCBI) 5080
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Background

Paired-box 6 (Pax6) is a highly conserved nuclear transcription factor that is key in neural tissue development, particularly eye morphogenesis.

What is the molecular weight of Pax6?

46.6 kDa. Pax6 is composed of 422 amino acids and contains a paired domain and a homeodomain.

What is the role of Pax6 in development?

In embryos, Pax6 is expressed in the developing central nervous system (CNS), eye, nose, and also in the pancreas. As a transcription factor, Pax6 is one of the earliest genes expressed in the eye and regulates the expression of many genes required for retinal and olfactory development (PMID: 10461206). The broad expression of Pax6 in the neuroectoderm, which develops to the CNS, makes it a useful marker for neural stem cells (PMID: 20621053).

The homology of Pax6 to the Drosophila gene eyeless and the mouse gene Small eye highlights its importance across a wide spectrum of organisms. In model organisms such as flies or mice, a dramatic phenotype can be seen. Mutations in eyeless lead to ectopic eye development and mice with heterozygote small eye mutations do not develop eyes or nasal cavities, and homozygote small eye mice do not survive (PMID: 7892602; PMID 8875254).

What is the role of Pax6 in eye disease?

Due to its key role as a master regulator in eye development, mutations in Pax6 have been associated with many different eye diseases. In humans, premature stop signals that lead to the production of a truncated protein have been found to cause aniridia, the absence of an iris (PMID: 19898691). Single amino acid mutations in Pax6 may lead to Peters' anomaly, which causes thinning and clouding of the cornea (PMID: 8162071).


Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for PAX6 antibody 12323-1-APProtokoll herunterladen
IHC protocol for PAX6 antibody 12323-1-APProtokoll herunterladenl
IF protocol for PAX6 antibody 12323-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
IF

Cell Stem Cell

Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis.

Authors - Miller Huang
mouseWB

Cell Stem Cell

Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells.

Authors - Mingzhu Yang
mouseWB

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
humanIF

Nat Commun

Reliability of high-quantity human brain organoids for modeling microcephaly, glioma invasion and drug screening

Authors - Anand Ramani
humanWB,IF

Cell Rep Med

End binding-3 inhibitor activates regenerative program in age-related macular degeneration

Authors - Quinn Lee
mouseWB

Nat Commun

ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling.

Authors - Xiaoyun Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kedar (Verified Customer) (08-28-2025)

Works well with NPCs and neurons derived from iPSCs

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: NPC and Neurons
FH

Christoffer (Verified Customer) (03-03-2025)

Works on adult mouse retina embedded in paraffin as well as cryo embedded postnatal (p7) mouse tissue.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: Adult mouse retina
FH

Ryszard (Verified Customer) (01-19-2024)

Antibody Pax-6 Rabbit works very well on whole mount mESC-derived cerebral organoids.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: mESC-derived Cerebral Organoids
PAX6 Antibody Immunofluorescence validation (1/500 dilution) in mESC-derived Cerebral Organoids (Cat no:12323-1-AP)
FH

Julie (Verified Customer) (01-18-2021)

This antibody works very well for immunofluorescence on human brain.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Human
FH

Rachel (Verified Customer) (02-01-2019)

Worked well and Proteintech employees were very helpful during the ordering process.

  • Applications: Other,
  • Cell Tissue Type: Epithelial cells