PAX6 Monoklonaler Antikörper

PAX6 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

CloneNo.

2C5A1

Rezensionen (1)

Kat-Nr. : 67529-1-Ig

Synonyme

2C5A1, paired box 6, Paired box protein Pax 6, Paired box protein Pax-6, PAX 6

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  WB analysis using 67529-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat cerebellum using 67529-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67529-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67529-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of JAR using 67529-1-Ig

  JAR cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of hTERT-RPE1 using 67529-1-Ig

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67529-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 67529-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A-673 cells, HeLa-Zellen, hTERT-RPE1-Zellen, JAR-Zellen, K-562-Zellen, Maus-Retina-Gewebe, Neuro-2a-Zellen, Kaninchen-Retina-Gewebe, Ratten-Cerebellum-Gewebe, Ratten-Retina-Gewebe, SW480-Zellen
• Erfolgreiche Detektion in IHC: Maus-Augengewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 7 publications below

Produktinformation

67529-1-Ig bindet in WB, IHC, IF, ELISA PAX6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PAX6 fusion protein Ag29977
• Vollständiger Name: paired box 6
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC011953
• Gene symbol: PAX6
• Gene ID (NCBI): 5080
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.

Publikationen

Species	Application	Title
human	WB	Sci Rep; Genome editing in spinocerebellar ataxia type 3 cells improves Golgi apparatus structure; Authors - Yanlin Wang
human	IF	Anal Bioanal Chem; Simultaneous and quantitative monitoring transcription factors in human embryonic stem cell differentiation using mass spectrometry-based targeted proteomics.; Authors - Mengying Xu
human	IF	Stem Cell Res; Generation of KCNH2 heterozygous knockout induced pluripotent stem cell (iPSC) line (Long and Short QT Syndrome); Authors - Baiqiang Wang
rat	IF	Neural Regen Res; Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions; Authors - Yuxue Mu
human	IF	Stem Cell Res; Generation of an induced pluripotent stem cell line (HMSCATi004-A) from an early onset Parkinson's disease patient with PRKN gene mutation; Authors - Jingke Cheng
human	IF	Stem Cell Res; Generation of heterozygous KCNA2 knockout induced pluripotent stem cell line; Authors - Wei Shan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Raquel (Verified Customer) (07-12-2024)

Immunofluorescence analysis in cryostat sections of 4% PFA fixed human brain organoid derived from iPSCs with 67529-1-Ig PAX6 antibody at dilution of 1:100 (under 20x lens). Antigen retrieval with Citrate buffer 10mM pH6. Green:Pax6 ; Blue: DAPI. Although most of the 67529-1-Ig immunostained cells colocalize with Pax6 positive cells, few of them seem to not colocalize.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human brain organoid

PAX6 Antibody Immunofluorescence validation (1:100 dilution) in Human brain organoid (Cat no:67529-1-Ig)