PAX6 Monoklonaler Antikörper

PAX6 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C5A1

Kat-Nr. : 67529-1-PBS

Synonyme

2C5A1, paired box 6, Paired box protein Pax 6, Paired box protein Pax-6, PAX 6

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  WB analysis using 67529-1-Ig (same clone as 67529-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

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  WB analysis of rat cerebellum using 67529-1-Ig (same clone as 67529-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67529-1-Ig (same clone as 67529-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67529-1-Ig (same clone as 67529-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

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  WB analysis of JAR using 67529-1-Ig (same clone as 67529-1-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

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  WB analysis of hTERT-RPE1 using 67529-1-Ig (same clone as 67529-1-PBS)

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67529-1-Ig (same clone as 67529-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 67529-1-Ig (same clone as 67529-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 67529-1-Ig (same clone as 67529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 67529-1-Ig (same clone as 67529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67529-1-Ig (same clone as 67529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67529-1-Ig (same clone as 67529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67529-1-Ig (same clone as 67529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67529-1-Ig (same clone as 67529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67529-1-PBS bindet in WB, IHC, Indirect ELISA PAX6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PAX6 fusion protein Ag29977
• Vollständiger Name: paired box 6
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC011953
• Gene symbol: PAX6
• Gene ID (NCBI): 5080
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.